Journal:

Article Title: The orphan nuclear receptor ROR? is a negative regulator of the inflammatory response

doi: 10.1093/embo-reports/kve007

Figure Lengend Snippet: Fig. 1. RORα is expressed in different vascular SMC types. (A and B) RT–PCR (35 cycles) analysis of RORα and GAPDH mRNA. C-PCR and C-RT are negative controls for PCR and RT, respectively; VSMC, smooth muscle cells from saphenous veins; CASMC, human coronary artery smooth muscle cells; HASMC, human aortic smooth muscle cells. (C) RT–PCR (30 cycles) analysis of RORα mRNA in SMC infected with Ad-RORα1 or Ad-GFP for 24 h. (D) Analysis of RORα protein expression in SMC infected for 24 h with or without Ad-RORα1. Immunocytochemistry experiments were performed as previously described (Chinetti et al., 1998) using a rabbit polyclonal RORα antibody raised against aa 163–225.

Article Snippet: Primary human aortic (HA) and coronary artery (CA) SMC (PromoCell, Heidelberg, Germany) and primary SMC from saphenous veins (VSMC: a kind gift of Dr Walsh, Boston, MA) were cultured under standard conditions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Infection, Expressing, Immunocytochemistry

Journal: Cells

Article Title: Macrophage-Derived Exosomal MALAT1 Induced by Hyperglycemia Regulates Vascular Calcification Through miR-143-3p/MGP Axis in Cultured Vascular Smooth Muscle Cells and Diabetic Rat Carotid Artery

doi: 10.3390/cells14241995

Figure Lengend Snippet: Effect of high glucose on exosomal MALAT1 expression in cultured macrophages and vascular smooth muscle cells (VSMCs). ( A ) Treatment with different glucose concentrations for 1 h. ( B ) Treatment with a glucose concentration of 25 mM for different periods of time. * p < 0.01 vs. control. ** p < 0.001 vs. control. N = 4 per group.

Article Snippet: Vascular smooth muscle cells (VSMCs) were obtained from PromoCell GmbH (Order No. c-12,511; Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Concentration Assay, Control

Journal: Physiological Reports

Article Title: Regulation of cell proliferation by the guanosine–adenosine mechanism: role of adenosine receptors

doi: 10.1002/phy2.24

Figure Lengend Snippet: Effects of guanosine on [ 3 H]-thymidine incorporation in human coronary artery vascular smooth muscle cells in the absence and presence of adenosine. Values represent means and SEMs. Two-factor ANOVA indicated a significant interaction between guanosine and adenosine on [ 3 H]-thymidine incorporation ( P < 0.000001). Letter “a” indicates a significant inhibitory response to guanosine at the indicated concentration of adenosine, and “b” indicates that the inhibitory response to guanosine is significantly greater in the presence of adenosine.

Article Snippet: Human coronary artery smooth muscle cells were obtained from Lonza Inc. (Allendale, NJ).

Techniques: Concentration Assay

Journal: Physiological Reports

Article Title: Regulation of cell proliferation by the guanosine–adenosine mechanism: role of adenosine receptors

doi: 10.1002/phy2.24

Figure Lengend Snippet: Effects of adenosine on [ 3 H]-thymidine incorporation in human coronary artery vascular smooth muscle cells in the absence (left panel) and presence (right panel) of guanosine, both without (no DPSPX; top graph) and with (pretreated with DPSPX; bottom graph) 1,3-dipropyl-8-( p -sulfophenyl)xanthine (DPSPX; 100 μmol/L; adenosine receptor antagonist). Values represent means and SEMs. Two-factor ANOVA indicated a significant interaction between guanosine and adenosine on [ 3 H]-thymidine incorporation in the “no DPSPX” group ( P < 0.000001) but not in the “pretreated with DPSPX” group. Letter “a” indicates significantly different from corresponding group without adenosine, and “b” indicates significantly different from corresponding “no guanosine” group.

Article Snippet: Human coronary artery smooth muscle cells were obtained from Lonza Inc. (Allendale, NJ).

Techniques:

Journal: Diabetologia

Article Title: Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice

doi: 10.1007/s00125-011-2241-2

Figure Lengend Snippet: Expression and abundance of GLP-1R and GIPR in monocytes/macrophages and arterial SMCs. a Glp1r and ( b ) Gipr mRNA in exudate peritoneal macrophages and tissues as labelled from non-treated Apoe −/− mice were measured by real-time RT-PCR. c Western blotting analyses of GLP-1R and ( d ) GIPR abundance in exudate peritoneal macrophages of non-treated Apoe −/− mice and other mouse tissues as labelled. Three independent experiments were performed. e Immunostaining of GLP-1R or GIPR in exudate peritoneal macrophages from non-treated Apoe −/− mice. Green, GLP-1R or GIPR as indicated; red, nuclei; red + green, overlay of GLP-1R or GIPR and nuclei. Scale bars 50 μm. f GLP1R and ( g ) GIPR mRNA levels in human coronary artery SMCs, THP1 cells and THP1-derived macrophages. h GIPR mRNA level in human monocytes and monocyte-derived macrophages were measured by real-time RT-PCR. Results were obtained from three to six independent experiments

Article Snippet: The expression of GIPR mRNA in human monocytes was far higher than in human macrophages and human coronary artery SMCs (Lonza) (Fig. ).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunostaining, Derivative Assay